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- 價格￥1000
- 規(guī)格100ul
- 貨號HKZ150309
- 單位支
- 品牌HaoKebio
產(chǎn)品介紹
產(chǎn)品參數(shù)
資料下載
| 品牌 | 商品編碼 | 商品名稱 | 規(guī)格 | 單位 | 單價 |
|---|
| HaoKebio | HKZ150309 | Oct-3/4兔單克隆抗體 | 100ul | 支 | 1000 |
|---|
Oct-3/4
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50186?Clone# ?BP6164
Predicted?Molecular Wt:
38.5kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q01860
Background:
OCT-4, also?known?as?OTF3 or?POU5F1,?is?a?member?of?the?POU?family?of?transcription?factors,?involved?in?the?regulation?of pluripotency during?normal development and?is detectable?in?embryonic?stem?and?germ?cells.?It?can?specifically?bind?to?the??octamer?motif?(5′-ATTTCAT-3′), and?it?is?critical?for?the?self-renewal?of?embryonic?stem?cells. OCT-4?is?a?key?regulator?of?self-?renewal?in embryonic stem cells;?its expression?is?potentially?correlated with?tumorigenesis?and?can?affect?some?aspects?of???tumor?behavior such as tumor?recurrence or?resistance to therapies.??OCT-4?is?a?sensitive?and?specific?marker for?germ?cell????tumors.?It?is consistently detected?in carcinoma?in situ/gonadoblastoma,?seminomas,?germinoma,?dysgerminoma,?and
embryonal carcinoma?but?not?in the differentiated components of?nonseminomas.?An?antibody?to?OCT-4?is?useful?in?the?identification of?primary as well as?metastatic germ?cell?tumors.
Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues?in?Human?Oct-3/4 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Zhang?S??et?al.?Nucleic?Acids?Res?47:4449-4461?(2019).
2.?Chang?YC??et?al.?Cell?Death?Dis?11:195?(2020).
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis
of??human seminoma
tissue?labelling?Oct-3/4?with?BP6164.?Heat
mediated?antigen
retrieval was?performed?using Tris/EDTA?buffer????pH?9.0
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